2sns
From Proteopedia
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STAPHYLOCOCCAL NUCLEASE. PROPOSED MECHANISM OF ACTION BASED ON STRUCTURE OF ENZYME-THYMIDINE 3(PRIME),5(PRIME)-BIPHOSPHATE-CALCIUM ION COMPLEX AT 1.5-ANGSTROMS RESOLUTION
Overview
The structure of the staphylococcal nuclease (EC 3.1.4.7)-thymidine, 3',5'-bisphosphate-Ca(2+) (enzyme-inhibitor) complex has been extended to, 1.5-A resolution by using much additional data and a phase refinement, scheme based on an electron-density map modification procedure. By, correlating this structure with the known properties of the enzyme, a, mechanism of action is proposed that involves nucleophilic attack on, phosphorus by a water molecule, which is bound to Glu-43, in line with the, 5'-CH(2)O(H) leaving group. The carboxylate of Glu-43 promotes this attack, by acting as a general base for the abstraction of a proton from the, attacking water molecule. Nucleophilic attack is further facilitated by, polarization of the phosphodiester by an ionic interaction between a, Ca(2+) ion and a phosphate oxygen atom and by four hydrogen bonds to, phosphate oxygen atoms from guanidinium ions of Arg-35 and Arg-87. These, interactions may also catalyze the reaction by lowering the energy of a, trigonal bipyramidal transition state. The hydrolysis of nucleic acid, substrate proceeds by cleavage of the 5'-P-O bond to yield a free, 5'-hydroxyl group and a terminal, 3'-phosphate monoester group. In the, inhibitor complex the only general acid group found in a position to, donate a proton to the leaving 5'-oxygen is the guanidinium ion of Arg-87., Alternative proton donors, presently lacking direct structural support, could be the phenolic hydroxyl group of Tyr-113 or a water molecule. The, precision and rigidity of the location of the reactants at the active site, and the probable dual binding and catalytic roles of the guanidinium ions, of Arg-35 and Arg-87 are especially noteworthy.
About this Structure
2SNS is a Single protein structure of sequence from Staphylococcus aureus with CA and THP as ligands. This structure superseeds the now removed PDB entry 1SNS. Active as Hydrolase, with EC number 3.1.33.1 Full crystallographic information is available from OCA.
Reference
Staphylococcal nuclease: proposed mechanism of action based on structure of enzyme-thymidine 3',5'-bisphosphate-calcium ion complex at 1.5-A resolution., Cotton FA, Hazen EE Jr, Legg MJ, Proc Natl Acad Sci U S A. 1979 Jun;76(6):2551-5. PMID:288045
Page seeded by OCA on Wed Nov 21 14:02:25 2007
