111d
From Proteopedia
|
CRYSTAL STRUCTURE AND STABILITY OF A DNA DUPLEX CONTAINING A(ANTI).G(SYN) BASE-PAIRS
Overview
The synthetic dodecanucleotide d(CGCAAATTGGCG) has been analysed by, single-crystal X-ray diffraction techniques and the structure refined to R, = 0.16 and 2.25 A resolution, with the location of 94 solvent molecules., The sequence crystallizes as a full turn of a B-DNA helix with ten, Watson-Crick base-pairs and two adenine-guanine mispairs. The analysis, clearly shows that the mismatches are of the form A(anti).G(syn). Thermal, denaturation studies indicate that the stability of the duplex is strongly, pH dependent, with a maximum at pH 5.0, suggesting that the base-pair is, stabilized by protonation. Three different arrangements have been observed, for base-pairs between guanine and adenine and it is likely that A.G, mismatch conformation is strongly influenced by dipole-dipole interactions, with adjacent base-pairs.
About this Structure
111D is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Crystal structure and stability of a DNA duplex containing A(anti).G(syn) base-pairs., Brown T, Leonard GA, Booth ED, Chambers J, J Mol Biol. 1989 May 20;207(2):455-7. PMID:2754734
Page seeded by OCA on Sat Nov 24 21:44:44 2007
