1qza
From Proteopedia
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Coordinates of the A/T site tRNA model fitted into the cryo-EM map of EF-Tu ternary complex (GDP.Kirromycin) bound 70S ribosome
Overview
Aminoacyl-tRNAs (aa-tRNAs) are delivered to the ribosome as part of the, ternary complex of aa-tRNA, elongation factor Tu (EF-Tu) and GTP. Here, we, present a cryo-electron microscopy (cryo-EM) study, at a resolution of, approximately 9 A, showing that during the incorporation of the aa-tRNA, into the 70S ribosome of Escherichia coli, the flexibility of aa-tRNA, allows the initial codon recognition and its accommodation into the, ribosomal A site. In addition, a conformational change observed in the, GTPase-associated center (GAC) of the ribosomal 50S subunit may provide, the mechanism by which the ribosome promotes a relative movement of the, aa-tRNA with respect to EF-Tu. This relative rearrangement seems to, facilitate codon recognition by the incoming aa-tRNA, and to provide the, codon-anticodon recognition-dependent signal for the GTPase activity of, EF-Tu. From these new findings we propose a mechanism that can explain the, sequence of events during the decoding of mRNA on the ribosome.
About this Structure
1QZA is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Incorporation of aminoacyl-tRNA into the ribosome as seen by cryo-electron microscopy., Valle M, Zavialov A, Li W, Stagg SM, Sengupta J, Nielsen RC, Nissen P, Harvey SC, Ehrenberg M, Frank J, Nat Struct Biol. 2003 Nov;10(11):899-906. Epub 2003 Oct 19. PMID:14566331
Page seeded by OCA on Sat Nov 24 21:50:12 2007
Categories: Protein complex | Ehrenberg, M. | Frank, J. | Harvey, S.C. | Li, W. | Nielsen, R.C. | Nissen, P. | Sengupta, J. | Stagg, S.M. | Valle, M. | Zavialov, A. | A/t-site trna. | Decoding | Trna model
