1vbk
From Proteopedia
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Crystal structure of PH1313 from Pyrococcus horikoshii Ot3
Overview
The putative thiamine-biosynthesis protein PH1313 from Pyrococcus, horikoshii OT3 has been overexpressed and purified. Crystallization was, performed by the oil-microbatch method using 28%(v/v), 2-methyl-2,4-pentanediol as a precipitant at 291 K. A native X-ray, diffraction data set at 1.9 A resolution and a single anomalous dispersion, data set from a selenomethionine-derivative crystal at 2.1 A resolution, were collected using synchrotron radiation at 100 K. The native crystal, belongs to the orthorhombic space group P2(1)2(1)2(1), with unit-cell, parameters a = 71.7, b = 71.2, c = 141.8 A.
About this Structure
1VBK is a Single protein structure of sequence from Pyrococcus horikoshii with MRD as ligand. Full crystallographic information is available from OCA.
Reference
Purification, crystallization and preliminary crystallographic analysis of the putative thiamine-biosynthesis protein PH1313 from Pyrococcus horikoshii OT3., Sugahara M, Murai S, Sugahara M, Kunishima N, Acta Crystallograph Sect F Struct Biol Cryst Commun. 2007 Jan 1;63(Pt, 1):56-8. Epub 2006 Dec 22. PMID:17183176
Page seeded by OCA on Sat Nov 24 22:02:21 2007
