This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1vbk
From Proteopedia
|
Crystal structure of PH1313 from Pyrococcus horikoshii Ot3
Overview
The putative thiamine-biosynthesis protein PH1313 from Pyrococcus, horikoshii OT3 has been overexpressed and purified. Crystallization was, performed by the oil-microbatch method using 28%(v/v), 2-methyl-2,4-pentanediol as a precipitant at 291 K. A native X-ray, diffraction data set at 1.9 A resolution and a single anomalous dispersion, data set from a selenomethionine-derivative crystal at 2.1 A resolution, were collected using synchrotron radiation at 100 K. The native crystal, belongs to the orthorhombic space group P2(1)2(1)2(1), with unit-cell, parameters a = 71.7, b = 71.2, c = 141.8 A.
About this Structure
1VBK is a Single protein structure of sequence from Pyrococcus horikoshii with MRD as ligand. Full crystallographic information is available from OCA.
Reference
Purification, crystallization and preliminary crystallographic analysis of the putative thiamine-biosynthesis protein PH1313 from Pyrococcus horikoshii OT3., Sugahara M, Murai S, Sugahara M, Kunishima N, Acta Crystallograph Sect F Struct Biol Cryst Commun. 2007 Jan 1;63(Pt, 1):56-8. Epub 2006 Dec 22. PMID:17183176
Page seeded by OCA on Sat Nov 24 22:02:21 2007
