1vck

From Proteopedia

proteopedia linkproteopedia link

spinqualitylabelsall models 1vck, resolution 1.90Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Crystal structure of ferredoxin component of carbazole 1,9a-dioxygenase of Pseudomonas resinovorans strain CA10

Overview

The carbazole 1,9a-dioxygenase (CARDO) system of Pseudomonas resinovorans, strain CA10 catalyzes the dioxygenation of carbazole; the 9aC carbon bonds, to a nitrogen atom and its adjacent 1C carbon as the initial reaction in, the mineralization pathway. The CARDO system is composed of ferredoxin, reductase (CarAd), ferredoxin (CarAc), and terminal oxygenase (CarAa)., CarAc acts as a mediator in the electron transfer from CarAd to CarAa. To, understand the structural basis of the protein-protein interactions during, electron transport in the CARDO system, the crystal structure of CarAc was, determined at 1.9 A resolution by molecular replacement using the, structure of BphF, the biphenyl 2,3-dioxygenase ferredoxin from, Burkholderia cepacia strain LB400 as a search model. CarAc is composed of, three beta-sheets, and the structure can be divided into two domains, a, cluster-binding domain and a basal domain. The Rieske [2Fe-2S] cluster is, located at the tip of the cluster-binding domain, where it is exposed to, solvent. While the overall folding of CarAc and BphF is strongly, conserved, the properties of their surfaces are very different from each, other. The structure of the cluster-binding domain of CarAc is more, compact and protruding than that of BphF, and the distribution of electric, charge on its molecular surface is very different. Such differences are, thought to explain why these ferredoxins can act as electron mediators in, respective electron transport chains composed of different-featured, components.

About this Structure

1VCK is a Single protein structure of sequence from Pseudomonas resinovorans with FE, FES and S as ligands. Full crystallographic information is available from OCA.

Reference

Crystal structure of the ferredoxin component of carbazole 1,9a-dioxygenase of Pseudomonas resinovorans strain CA10, a novel Rieske non-heme iron oxygenase system., Nam JW, Noguchi H, Fujimoto Z, Mizuno H, Ashikawa Y, Abo M, Fushinobu S, Kobashi N, Wakagi T, Iwata K, Yoshida T, Habe H, Yamane H, Omori T, Nojiri H, Proteins. 2005 Mar 1;58(4):779-89. PMID:15645447

Page seeded by OCA on Sat Nov 24 22:08:14 2007