1efs
From Proteopedia
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CONFORMATION OF A DNA-RNA HYBRID
Overview
RNA--DNA hybrid duplexes are involved in transcription, replication and, reverse transcription of nucleic acids. Information on such duplexes may, shed some light on the mechanism of these processes. For this purpose, the, influence of base composition on the structure of a, polypyrimidine--polypurine RNA--DNA duplex r(cucuccuucucuu)., d(GAGAGGAAGAGAA) has been studied using 1H, 31P and 13C NMR experiments, molecular modeling (JUMNA program) and NOE back-calculation methods. The, resulting structure of the 13-mer hybrid duplex shows that the RNA strand, is in the expected A-type conformation while the DNA strand is in a very, flexible conformation. In the DNA strand, the desoxyribose sugars retain, the C2'-endo B-type conformation. The duplex helical parameters (such as, inclination, twist and displacement of the bases) are close to the A-type, conformation. No bending was observed for the global axis curvature. The, major groove width is close to the B-form value and the minor groove width, is intermediate between standard values for A and B-forms. These results, are in favour of the independence of minor groove size (where RNase H, interacts) and the base composition of the hybrid duplexes.
About this Structure
1EFS is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Solution conformation of an RNA--DNA hybrid duplex containing a pyrimidine RNA strand and a purine DNA strand., Hantz E, Larue V, Ladam P, Le Moyec L, Gouyette C, Huynh Dinh T, Int J Biol Macromol. 2001 Apr 12;28(4):273-84. PMID:11311717
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