1n3p
From Proteopedia
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Pterocarpus angolensis lectin in complex with sucrose
Overview
The crystal structure of the Man/Glc-specific seed lectin from Pterocarpus, angolensis was determined in complex with methyl-alpha-d-glucose, sucrose, and turanose. The carbohydrate binding site contains a classic Man/Glc, type specificity loop. Its metal binding loop on the other hand is of the, long type, different from what is observed in other Man/Glc-specific, legume lectins. Glucose binding in the primary binding site is reminiscent, of the glucose complexes of concanavalin A and lentil lectin. Sucrose is, found to be bound in a conformation similar as seen in the binding site of, lentil lectin. A direct hydrogen bond between Ser-137(OG) to Fru(O2) in, Pterocarpus angolensis lectin replaces a water-mediated interaction in the, equivalent complex of lentil lectin. In the turanose complex, the binding, site of the first molecule in the asymmetric unit contains the, alphaGlc1-3betaFruf form of furanose while the second molecule contains, the alphaGlc1-3betaFrup form in its binding site.
About this Structure
1N3P is a Protein complex structure of sequences from Pterocarpus angolensis with MN and CA as ligands. Full crystallographic information is available from OCA.
Reference
Crystal structure of Pterocarpus angolensis lectin in complex with glucose, sucrose, and turanose., Loris R, Imberty A, Beeckmans S, Van Driessche E, Read JS, Bouckaert J, De Greve H, Buts L, Wyns L, J Biol Chem. 2003 May 2;278(18):16297-303. Epub 2003 Feb 19. PMID:12595543
Page seeded by OCA on Sat Nov 24 22:30:56 2007
