2boz

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spinqualitylabelsall models 2boz, resolution 2.40Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

PHOTOSYNTHETIC REACTION CENTER MUTANT WITH GLY M203 REPLACED WITH LEU

Overview

The role of a water molecule (water A) located between the primary, electron donor (P) and first electron acceptor bacteriochlorophyll (B(A)), in the purple bacterial reaction center was investigated by mutation of, glycine M203 to leucine (GM203L). The x-ray crystal structure of the, GM203L reaction center shows that the new leucine residue packs in such a, way that water A is sterically excluded from the complex, but the, structure of the protein-cofactor system around the mutation site is, largely undisturbed. The results of absorbance and resonance Raman, spectroscopy were consistent with either the removal of a hydrogen bond, interaction between water A and the keto carbonyl group of B(A) or a, change in the local electrostatic environment of this carbonyl group., Similarities in the ... [(full description)]

About this Structure

2BOZ is a [Protein complex] structure of sequences from [Rhodobacter sphaeroides] with FE, PO4, LDA, D10, BCL, BH1, U10 and SPN as [ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].

Reference

Strong effects of an individual water molecule on the rate of light-driven charge separation in the Rhodobacter sphaeroides reaction center., Potter JA, Fyfe PK, Frolov D, Wakeham MC, van Grondelle R, Robert B, Jones MR, J Biol Chem. 2005 Jul 22;280(29):27155-64. Epub 2005 May 20. PMID:15908429

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