1rmn
From Proteopedia
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A THREE-DIMENSIONAL MODEL FOR THE HAMMERHEAD RIBOZYME BASED ON FLUORESCENCE MEASUREMENTS
Overview
For the understanding of the catalytic function of the RNA hammerhead, ribozyme, a three-dimensional model is essential but neither a crystal nor, a solution structure has been available. Fluorescence resonance energy, transfer (FRET) was used to study the structure of the ribozyme in, solution in order to establish the relative spatial orientation of the, three constituent Watson-Crick base-paired helical segments. Synthetic, constructs were labeled with the fluorescence donor (5-carboxyfluorescein), and acceptor (5-carboxytetramethylrhodamine) located at the ends of the, strands constituting the ribozyme molecule. The acceptor helix in helix, pairs I and III and in II and III was varied in length from 5 to 11 and 5, to 9 base pairs, respectively, and the FRET efficiencies were determined, and correlated with a reference set of labeled RNA duplexes. The FRET, efficiencies were predicted on the basis of vector algebra analysis, as a, function of the relative helical orientations in the ribozyme constructs, and compared with experimental values. The data were consistent with a, Y-shaped arrangement of the ribozyme with helices I and II in close, proximity and helix III pointing away. These orientational constraints, were used for molecular modeling of a three-dimensional structure of the, complete ribozyme.
About this Structure
1RMN is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
A three-dimensional model for the hammerhead ribozyme based on fluorescence measurements., Tuschl T, Gohlke C, Jovin TM, Westhof E, Eckstein F, Science. 1994 Nov 4;266(5186):785-9. PMID:7973630
Page seeded by OCA on Sat Nov 24 22:56:18 2007
