1rna

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Image:1rna.gif

1rna, resolution 2.250Å

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CRYSTALLOGRAPHIC STRUCTURE OF AN RNA HELIX: [U(U-A)6A]2

Overview

The crystallographic structure of the synthetic oligoribonucleotide, U(UA)6A, has been solved at 2.25 A resolution. The crystallographic, refinement permitted the identification of 91 solvent molecules, with a, final agreement factor of 13%. The molecule is a dimer of 14 base-pairs, and shows the typical features of an A-type helix. However, the presence, of two kinks causes a divergence from a straight helix. The observed, deformation, which is stabilized by a few hydrogen bonds in the crystal, packing, could be due to the relatively high (35 degrees C) temperature of, crystallization. The complete analysis of the structure is presented. It, includes the stacking geometries, the backbone conformation and the, solvation.

About this Structure

1RNA is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.

Reference

Crystallographic structure of an RNA helix: [U(UA)6A]2., Dock-Bregeon AC, Chevrier B, Podjarny A, Johnson J, de Bear JS, Gough GR, Gilham PT, Moras D, J Mol Biol. 1989 Oct 5;209(3):459-74. PMID:2479753

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