This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1ndn
From Proteopedia
|
MOLECULAR STRUCTURE OF NICKED DNA. MODEL T4
Overview
The molecular structure of a nicked dodecamer DNA double helix, made of a, ternary system containing d(CGCGAAAACGCG) + d(CGCGTT) + d(TTCGCG), oligonucleotides, has been determined by x-ray diffraction analysis at 3 A, resolution. The molecule adopts a B-DNA conformation, not unlike those, found in intact dodecamer DNA molecules crystallized in a somewhat, different crystal lattice, despite a gap due to the absence of a phosphate, group in the molecule. The helix has a distinct narrow minor groove near, the center of the molecule at the AAAA region. This suggests that the, internal stabilizing forces due to base stacking and hydrogen-bonding, interactions are sufficient to overcome the loss of connectivity, associated with the disruption of the covalent backbone of DNA.
About this Structure
1NDN is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Molecular structure of nicked DNA: a substrate for DNA repair enzymes., Aymami J, Coll M, van der Marel GA, van Boom JH, Wang AH, Rich A, Proc Natl Acad Sci U S A. 1990 Apr;87(7):2526-30. PMID:2320572
Page seeded by OCA on Sat Nov 24 22:58:16 2007
