1f5j
From Proteopedia
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CRYSTAL STRUCTURE OF XYNB, A HIGHLY THERMOSTABLE BETA-1,4-XYLANASE FROM DICTYOGLOMUS THERMOPHILUM RT46B.1, AT 1.8 A RESOLUTION
Overview
Microorganisms employ a large array of enzymes to break down the cellulose, and hemicelluloses of plant biomass. These enzymes, especially those with, high thermal stability, have many uses in biotechnology. We have solved, the crystal structure of a beta-1, 4-xylanase, XynB, from the extremely, thermophilic bacterium Dictyoglomus thermophilum, isolate Rt46B.1. The, protein crystallized from 1.6 M ammonium sulfate, 0.2 M HEPES pH 7.2 and, 10% glycerol, with unit-cell parameters a = b = 91.3, c = 44.9 A and space, group P4(3). The structure was solved at high resolution (1.8 A) by X-ray, crystallography, using the method of isomorphous replacement with a single, mercury derivative, and refined to a final R factor of 18.3% (R(free) =, 22.1%). XynB has the single-domain fold typical of family 11 xylanases, comprising a jelly roll of two highly twisted beta-sheets that create a, deep substrate-binding cleft. The two catalytic residues, Glu90 and, Glu180, occupy this cleft. Compared with other family 11 xylanases, XynB, has a greater proportion of polar surface and has a slightly extended, C-terminus that, combined with the extension of beta-strand A5, gives, additional hydrogen bonding and hydrophobic packing. These factors may, account for the enhanced thermal stability of the enzyme.
About this Structure
1F5J is a Single protein structure of sequence from Dictyoglomus thermophilum with SO4 as ligand. Active as Endo-1,4-beta-xylanase, with EC number 3.2.1.8 Full crystallographic information is available from OCA.
Reference
Structure of XynB, a highly thermostable beta-1,4-xylanase from Dictyoglomus thermophilum Rt46B.1, at 1.8 A resolution., McCarthy AA, Morris DD, Bergquist PL, Baker EN, Acta Crystallogr D Biol Crystallogr. 2000 Nov;56(Pt 11):1367-75. PMID:11053833
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