1nm2
From Proteopedia
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"Malonyl-CoA:ACP Transacylase"
Overview
Malonyl-CoA:ACP transacylase (MAT), the fabD gene product of Streptomyces, coelicolor A3(2), participates in both fatty acid and polyketide synthesis, pathways, transferring malonyl groups that are used as extender units in, chain growth from malonyl-CoA to pathway-specific acyl carrier proteins, (ACPs). Here, the 2.0 A structure reveals an invariant arginine bound to, an acetate that mimics the malonyl carboxylate and helps define the, extender unit binding site. Catalysis may only occur when the oxyanion, hole is formed through substrate binding, preventing hydrolysis of the, acyl-enzyme intermediate. Macromolecular docking simulations with, actinorhodin ACP suggest that the majority of the ACP docking surface is, formed by a helical flap. These results should help to engineer polyketide, synthases (PKSs) that produce novel polyketides.
About this Structure
1NM2 is a Single protein structure of sequence from Bacteria with NI and ACY as ligands. Active as [Acyl-carrier-protein_S-malonyltransferase [Acyl-carrier-protein] S-malonyltransferase], with EC number 2.3.1.39 Full crystallographic information is available from OCA.
Reference
Catalysis, specificity, and ACP docking site of Streptomyces coelicolor malonyl-CoA:ACP transacylase., Keatinge-Clay AT, Shelat AA, Savage DF, Tsai SC, Miercke LJ, O'Connell JD 3rd, Khosla C, Stroud RM, Structure. 2003 Feb;11(2):147-54. PMID:12575934[[Category: [Acyl-carrier-protein] S-malonyltransferase]]
Page seeded by OCA on Sat Nov 24 23:22:55 2007
