1ntb
From Proteopedia
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2.9 A crystal structure of Streptomycin RNA-aptamer complex
Overview
We describe a 2.9 A X-ray structure of a complex between the aminocyclitol, antibiotic streptomycin and an in vitro selected RNA aptamer, solved using, the anomalous diffraction properties of Ba cations. The RNA aptamer, which, contains two asymmetric internal loops, adopts a distinct, cation-stabilized fold involving a series of S-shaped backbone turns, anchored by canonical and noncanonical pairs and triples. The streptomycin, streptose ring is encapsulated by stacked arrays of bases from both loops, at the elbow of the L-shaped RNA architecture. Specificity is defined by, direct hydrogen bonds between all streptose functional groups and base, edges that line the inner walls of the cylindrical binding pocket. By, contrast, the majority of intermolecular interactions involve contacts to, backbone phosphates in the published structure of streptomycin bound to, the 16S rRNA.
About this Structure
1NTB is a Protein complex structure of sequences from [1] with MG, NA and SRY as ligands. Full crystallographic information is available from OCA.
Reference
Encapsulating streptomycin within a small 40-mer RNA., Tereshko V, Skripkin E, Patel DJ, Chem Biol. 2003 Feb;10(2):175-87. PMID:12618190
Page seeded by OCA on Sat Nov 24 23:46:33 2007
