1wqz
From Proteopedia
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Complicated water orientations in the minor groove of B-DNA decamer D(CCATTAATGG)2 observed by neutron diffraction measurements
Overview
It has long been suspected that the structure and function of a DNA duplex, can be strongly dependent on its degree of hydration. By neutron, diffraction experiments, we have succeeded in determining most of the, hydrogen (H) and deuterium (D) atomic positions in the decameric, d(CCATTAATGG)2 duplex. Moreover, the D positions in 27 D2O molecules have, been determined. In particular, the complex water network in the minor, groove has been observed in detail. By a combined structural analysis, using 2.0 A resolution X-ray and 3.0 A resolution neutron data, it is, clear that the spine of hydration is built up, not only by a simple, hexagonal hydration pattern (as reported in earlier X-ray studies), but, also by many other water bridges hydrogen-bonded to the DNA strands. The, complexity of the hydration pattern in the minor groove is derived from an, extraordinary variety of orientations displayed by the water molecules.
About this Structure
1WQZ is a Protein complex structure of sequences from [1] with DOD as ligand. Full crystallographic information is available from OCA.
Reference
Complicated water orientations in the minor groove of the B-DNA decamer d(CCATTAATGG)2 observed by neutron diffraction measurements., Arai S, Chatake T, Ohhara T, Kurihara K, Tanaka I, Suzuki N, Fujimoto Z, Mizuno H, Niimura N, Nucleic Acids Res. 2005 May 24;33(9):3017-24. Print 2005. PMID:15914673
Page seeded by OCA on Sun Nov 25 00:34:19 2007
Categories: Protein complex | Arai, S. | Chatake, T. | Fujimoto, Z. | Kurihara, K. | Mizuno, H. | Niimura, N. | Ohhara, T. | Suzuki, N. | Tanaka, I. | DOD | B-dna | Hydration | Hydrogen | Neutron diffraction
