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1bwt
From Proteopedia
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NMR SOLUTION STRUCTURE OF [D(GCGAATCGC)2]
Overview
We present the high-resolution solution structures of a self-complementary, DNA decamer duplex featuring a single alpha-anomeric nucleotide per strand, encompassed by a set of 3'-3' and 5'-5' phosphodiester linkages, d(GCGAAT-3'-3'-alphaT-5'-5'-CGC)2, alphaT, and its unmodified control, d(GCGAATTCGC)2, obtained by restrained molecular dynamics. Interproton, distance and deoxyribose ring torsion angle restraints were deduced from, homonuclear NOESY and DQF-COSY data, respectively. For both the control, and alphaT duplexes, excellent global convergence was observed from two, different (A- and B-) starting models. The final average structures of the, two duplexes are highly homologous, and overall possess the traits, characteristic of right-handed B-DNA duplexes. However, localized, differences between the two structures stem from the enhanced, conformational exchange in the deoxyribose ring of the cytidine following, the 5'-5' linkage, the C3'- exo pseudorotation phase angle of the, alpha-nucleotide, and unusual backbone torsions in the 3'-3' and 5'-5', phosphodiester linkages. The structural data reported here are relevant to, the design of antisense therapeutics comprised of these modifications.
About this Structure
1BWT is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
NMR solution structures of [d(GCGAAT-3'-3'-alphaT-5'-5'-CGC)2] and its unmodified control., Aramini JM, Mujeeb A, Germann MW, Nucleic Acids Res. 1998 Dec 15;26(24):5644-54. PMID:9837995
Page seeded by OCA on Sun Nov 25 00:43:11 2007
