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1k6g
From Proteopedia
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Solution Structure of Conserved AGNN Tetraloops: Insights into Rnt1p RNA Processing
Overview
Rnt1p, the yeast orthologue of RNase III, cleaves rRNAs, snRNAs and, snoRNAs at a stem capped with conserved AGNN tetraloop. Here we show that, 9 bp long stems ending with AGAA or AGUC tetraloops bind to Rnt1p and, direct specific but sequence-independent RNA cleavage when provided with, stems longer than 13 bp. The solution structures of these two tetraloops, reveal a common fold for the terminal loop stabilized by non-canonical A-A, or A-C pairs and extensive base stacking. The conserved nucleotides are, stacked at the 5' side of the loop, exposing their Watson-Crick and, Hoogsteen faces for recognition by Rnt1p. These results indicate that, yeast RNase III recognizes the fold of a conserved single-stranded, tetraloop to direct specific dsRNA cleavage.
About this Structure
1K6G is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Solution structure of conserved AGNN tetraloops: insights into Rnt1p RNA processing., Lebars I, Lamontagne B, Yoshizawa S, Aboul-Elela S, Fourmy D, EMBO J. 2001 Dec 17;20(24):7250-8. PMID:11743001
Page seeded by OCA on Sun Nov 25 00:50:13 2007
