1ww9

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spinqualitylabelsall models 1ww9, resolution 1.95Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Crystal structure of the terminal oxygenase component of carbazole 1,9a-dioxygenase, a non-heme iron oxygenase system catalyzing the novel angular dioxygenation for carbazole and dioxin

Overview

Carbazole 1,9a-dioxygenase (CARDO) catalyzes the dihydroxylation of, carbazole by angular position (C9a) carbon bonding to the imino nitrogen, and its adjacent C1 carbon. This reaction is an initial degradation, reaction of the carbazole degradation pathway by various bacterial, strains. Only a limited number of Rieske non-heme iron oxygenase systems, (ROSs) can catalyze this novel reaction, termed angular dioxygenation., Angular dioxygenation is also involved in the degradation pathways of, carbazole-related compounds, dioxin, and CARDO can catalyze the angular, dioxygenation for dioxin. CARDO consists of a terminal oxygenase component, (CARDO-O), and the electron transport components, ferredoxin (CARDO-F) and, ferredoxin reductase (CARDO-R). CARDO-O has a homotrimeric structure, and, governs the substrate specificity of CARDO. Here, we have determined the, crystal structure of CARDO-O of Janthinobacterium sp. strain J3 at a, resolution of 1.95A. The alpha3 trimeric overall structure of the CARDO-O, molecule roughly corresponds to the alpha3 partial structures of other, terminal oxygenase components of ROSs that have the alpha3beta3, configuration. The CARDO-O structure is a first example of the terminal, oxygenase components of ROSs that have the alpha3 configuration, and, revealed the presence of the specific loops that interact with a, neighboring subunit, which is proposed to be indispensable for stable, alpha3 interactions without structural beta subunits. The shape of the, substrate-binding pocket of CARDO-O is markedly different from those of, other oxygenase components involved in naphthalene and biphenyl, degradation pathways. Docking simulations suggested that carbazole binds, to the substrate-binding pocket in a manner suitable for catalysis of, angular dioxygenation.

About this Structure

1WW9 is a Single protein structure of sequence from Janthinobacterium sp. j3 with FE2 and FES as ligands. Full crystallographic information is available from OCA.

Reference

Structure of the terminal oxygenase component of angular dioxygenase, carbazole 1,9a-dioxygenase., Nojiri H, Ashikawa Y, Noguchi H, Nam JW, Urata M, Fujimoto Z, Uchimura H, Terada T, Nakamura S, Shimizu K, Yoshida T, Habe H, Omori T, J Mol Biol. 2005 Aug 12;351(2):355-70. PMID:16005887

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