1st8
From Proteopedia
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Crystal structure of fructan 1-exohydrolase IIa from Cichorium intybus
Overview
Fructan 1-exohydrolase, an enzyme involved in fructan degradation, belongs, to the glycosyl hydrolase family 32. The structure of isoenzyme 1-FEH IIa, from Cichorium intybus is described at a resolution of 2.35 A. The, structure consists of an N-terminal fivefold beta-propeller domain, connected to two C-terminal beta-sheets. The putative active site is, located entirely in the beta-propeller domain and is formed by amino acids, which are highly conserved within glycosyl hydrolase family 32. The, fructan-binding site is thought to be in the cleft formed between the two, domains. The 1-FEH IIa structure is compared with the structures of two, homologous but functionally different enzymes: a levansucrase from, Bacillus subtilis (glycosyl hydrolase family 68) and an invertase from, Thermotoga maritima (glycosyl hydrolase family 32).
About this Structure
1ST8 is a Single protein structure of sequence from Cichorium intybus with GOL as ligand. Active as Fructan beta-fructosidase, with EC number 3.2.1.80 Full crystallographic information is available from OCA.
Reference
X-ray diffraction structure of a plant glycosyl hydrolase family 32 protein: fructan 1-exohydrolase IIa of Cichorium intybus., Verhaest M, Ende WV, Roy KL, De Ranter CJ, Laere AV, Rabijns A, Plant J. 2005 Feb;41(3):400-11. PMID:15659099
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