1c8n
From Proteopedia
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TOBACCO NECROSIS VIRUS
Overview
The crystal structure of tobacco necrosis virus (TNV) has been determined, by real-space averaging with 5-fold non-crystallographic symmetry, and, refined to R=25.3 % for diffraction data to 2.25 A resolution. A total of, 180 subunits form a T=3 virus shell with a diameter of about 280 A and a, small protrusion at the 5-fold axis. In 276 amino acid residues, the, respective amino terminal 86, 87 and 56 residues of the A, B and C, subunits are disordered. No density for the RNA was found. The subunits, have a "jelly roll" beta-barrel structure, as have the structures of the, subunits of other spherical viruses. The tertiary and quaternary, structures of TNV are, in particular, similar to those of southern bean, mosaic virus, although they are classified in different groups. Invisible, residues 1 to 56 with a high level of basic residues are considered to be, located inside the particle. Sequence comparison of the coat proteins of, several TNV strains showed that the sequences of the disordered segment, diverge considerably as compared with those of the ordered segment, consistent with a small tertiary structural constraint being imposed on, the N-terminal segment. Basic residues are localized on the subunit, interfaces or inner surface of the capsid. Positive charges of the basic, residues facing the interior, as well as those of the N-terminal segment, may neutralize the negative charge of the RNA inside. Five calcium ions, per icosahedral asymmetric unit are located at the subunit interfaces;, three are close to the exterior surface, the other two away from it. The, environments of the first three are similar, and those of the other two, sites are similar. These calcium ions are assumed to be responsible for, the stabilization/transition of the quaternary structure of the shell., Three peptide segments ordered only in the C subunits are clustered around, each 3-fold (quasi-6-fold) axis forming a beta-annulus, and may lead to, quasi-equivalent interactions for the organization of the T=3 shell.
About this Structure
1C8N is a Single protein structure of sequence from Unidentified tobacco necrosis virus with CA as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structure of tobacco necrosis virus at 2.25 A resolution., Oda Y, Saeki K, Takahashi Y, Maeda T, Naitow H, Tsukihara T, Fukuyama K, J Mol Biol. 2000 Jun 30;300(1):153-69. PMID:10864506
Page seeded by OCA on Sun Nov 25 01:15:27 2007
