1gjx
From Proteopedia
|
SOLUTION STRUCTURE OF THE LIPOYL DOMAIN OF THE CHIMERIC DIHYDROLIPOYL DEHYDROGENASE P64K FROM NEISSERIA MENINGITIDIS
Overview
The antigenic P64K protein from the pathogenic bacterium Neisseria, meningitidis is found in the outer membrane of the cell, and consists of, two parts: an 81-residue N-terminal region and a 482-residue C-terminal, region. The amino-acid sequence of the N-terminal region is homologous, with the lipoyl domains of the dihydrolipoyl acyltransferase (E2), components, and that of the C-terminal region with the dihydrolipoyl, dehydrogenase (E3) components, of 2-oxo acid dehydrogenase multienzyme, complexes. The two parts are separated by a long linker region, similar to, the linker regions in the E2 chains of 2-oxo acid dehydrogenase complexes, and it is likely this region is conformationally flexible. A subgene, encoding the P64K lipoyl domain was created and over-expressed in, Escherichia coli. The product was capable of post-translational, modification by the lipoate protein ligase but not aberrant modification, by the biotin protein ligase of E. coli. The solution structure of the, apo-domain was determined by means of heteronuclear NMR spectroscopy and, found to be a flattened beta barrel composed of two four-stranded, antiparallel beta sheets. The lysine residue that becomes lipoylated is in, an exposed beta turn that, from a [1H]-15N heteronuclear Overhauser effect, experiment, appears to enjoy substantial local motion. This structure of a, lipoyl domain derived from a dihydrolipoyl dehydrogenase resembles that of, lipoyl domains normally found as part of the dihydrolipoyl acyltransferase, component of 2-oxo acid dehydrogenase complexes and will assist in, furthering the understanding of its function in a multienzyme complex and, in the membrane-bound P64K protein itself.
About this Structure
1GJX is a Single protein structure of sequence from Neisseria meningitidis. Active as Dihydrolipoyl dehydrogenase, with EC number 1.8.1.4 Full crystallographic information is available from OCA.
Reference
Solution structure of the lipoyl domain of the chimeric dihydrolipoyl dehydrogenase P64K from Neisseria meningitidis., Tozawa K, Broadhurst RW, Raine AR, Fuller C, Alvarez A, Guillen G, Padron G, Perham RN, Eur J Biochem. 2001 Sep;268(18):4908-17. PMID:11559360
Page seeded by OCA on Sun Nov 25 01:28:00 2007
