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Publication Abstract from PubMed

Rosellinia necatrix megabirnavirus 1-W779 (RnMBV1) is a non-enveloped icosahedral double-stranded (ds)RNA virus that infects the ascomycete fungus Rosellinia necatrix, a causative agent that induces a lethal plant disease white root rot. Herein, we have first resolved the atomic structure of the RnMBV1 capsid at 3.2 A resolution using cryo-electron microscopy (cryo-EM) single-particle analysis. Compared with other non-enveloped icosahedral dsRNA viruses, the RnMBV1 capsid protein structure exhibits an extra-long C-terminal arm and a surface protrusion domain. In addition, the previously unrecognized crown proteins are identified in a symmetry-expanded cryo-EM model and are present over the 3-fold axes. These exclusive structural features of the RnMBV1 capsid could have been acquired for playing essential roles in transmission and/or particle assembly of the megabirnaviruses. Our findings, therefore, will reinforce the understanding of how the structural and molecular machineries of the megabirnaviruses influence the virulence of the disease-related ascomycete fungus.

Capsid structure of a fungal dsRNA megabirnavirus reveals its previously unidentified surface architecture.,Wang H, Salaipeth L, Miyazaki N, Suzuki N, Okamoto K PLoS Pathog. 2023 Feb 27;19(2):e1011162. doi: 10.1371/journal.ppat.1011162. , eCollection 2023 Feb. PMID:36848381^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.