2zvr

Structural highlights

Function

IOLO_THEMA Catalyzes the reversible epimerization between 5-keto-L-gluconate (5-dehydro-L-gluconate) and D-tagaturonate, and thus probably functions in a myo-inositol degradation pathway together with IolG, IolM and IolN (PubMed:23441918). Is not active on the enantiomer 5-keto-D-gluconate (PubMed:23441918). Was also shown to be a nonphosphorylated sugar isomerase with broad substrate specificity in vitro (PubMed:28258150). Is able to catalyze the reversible C3-epimerization of L-ribulose to L-xylulose, D-ribulose to D-xylulose, D-psicose to D-fructose, and D-tagatose to D-sorbose, with a substrate preference for ketopentoses rather than ketohexoses (PubMed:28258150). Also catalyzes the aldose-ketose isomerization reaction from either D-erythrose or D-threose to D-erythrulose (PubMed:28258150). Exhibits no activity for C4-epimerization of D-tagatose to D-fructose (PubMed:28258150).^{[1] [2]}

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

References

1. ↑ Rodionova IA, Leyn SA, Burkart MD, Boucher N, Noll KM, Osterman AL, Rodionov DA. Novel inositol catabolic pathway in Thermotoga maritima. Environ Microbiol. 2013 Aug;15(8):2254-66. PMID:23441918 doi:10.1111/1462-2920.12096
2. ↑ Shin SM, Cao TP, Choi JM, Kim SB, Lee SJ, Lee SH, Lee DW. TM0416 Is a Hyperthermophilic Promiscuous Non-phosphorylated Sugar Isomerase That Catalyzes Various C5 and C6 Epimerization Reactions. Appl Environ Microbiol. 2017 Mar 3. pii: AEM.03291-16. doi: 10.1128/AEM.03291-16. PMID:28258150 doi:http://dx.doi.org/10.1128/AEM.03291-16