3epv
From Proteopedia
X-ray Structure of the Metal-sensor CnrX in both the Apo- and Copper-bound Forms
Structural highlights
FunctionCNRR_CUPMC CnrH alone is able to activate cnr expression, while both CnrY and CrnR (CnrX) are needed for nickel induction of CnrH (PubMed:10671463). Has been suggested (PubMed:10671464) to bind nickel.[1] [2] Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedBoth the X-ray structures of the apo- and the copper-bound forms of the metal-sensor domain (residues 31-148) of CnrX from Cupriavidus metallidurans CH34 were obtained at 1.74A resolution from a selenomethionine derivative. This four-helix hooked-hairpin is the first structure of a metal-sensor in an ECF-type signaling pathway. The copper ion is bound in a type 2-like center with a 3N1O coordination in the equatorial plane and shows an unprecedented remote fifth axial ligand with Met93 contributing a weak S-Cu bond. The signal onset cannot be explained by conformational changes associated with CnrX metallation. X-ray structure of the metal-sensor CnrX in both the apo- and copper-bound forms.,Pompidor G, Maillard AP, Girard E, Gambarelli S, Kahn R, Coves J FEBS Lett. 2008 Nov 26;582(28):3954-8. Epub 2008 Nov 4. PMID:18992246[3] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
| ||||||||||||||||||||
