4m7m

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Crystal structure of the R111K:R132Q:Y134F:T54V:R59W:A32W mutant of the Cellular Retinoic Acid Binding Protein Type II in complex with All-Trans Retinal at 2.57 Angstrom Resolution

Structural highlights

4m7m is a 1 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.571Å
Ligands:RET
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RABP2_HUMAN Transports retinoic acid to the nucleus. Regulates the access of retinoic acid to the nuclear retinoic acid receptors.

Publication Abstract from PubMed

Reengineering of Cellular Retinoic Acid Binding Protein II (CRABPII) to be capable of binding retinal as a protonated Schiff base is described. Through rational alterations of the binding pocket, electrostatic perturbations of the embedded retinylidene chromophore that favor charge delocalization of the iminium charge lead to exquisite control in the regulation of chromophoric absorption properties, spanning the visible spectrum (474-640 nm). The pKa of the retinylidine protonated Schiff base was modulated from 2.4 to 8.1, giving rise to a set of proteins of varying colors and pH sensitivities. These proteins were used to demonstrate a concentration independent, ratiometric pH sensor.

Rational Design of a Colorimetric pH Sensor from a Soluble Retinoic Acid Chaperone.,Berbasova T, Nosrati M, Vasileiou C, Wang W, Lee KS, Yapici I, Geiger JH, Borhan B J Am Chem Soc. 2013 Sep 20. PMID:24059243[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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See Also

References

  1. ↑ Berbasova T, Nosrati M, Vasileiou C, Wang W, Lee KS, Yapici I, Geiger JH, Borhan B. Rational Design of a Colorimetric pH Sensor from a Soluble Retinoic Acid Chaperone. J Am Chem Soc. 2013 Sep 20. PMID:24059243 doi:http://dx.doi.org/10.1021/ja404900k

Contents


PDB ID 4m7m

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OCA

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