5o7b

From Proteopedia

CRYSTAL STRUCTURE OF THE SLR0328 TYROSINE PHOSPHATASE WZB FROM SYNECHOCYSTIS SP. PCC 6803

Structural highlights

5o7b is a 1 chain structure with sequence from Synechocystis sp. PCC 6803 substr. Kazusa. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.281Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Y328_SYNY3

Publication Abstract from PubMed

Many cyanobacteria produce extracellular polymeric substances (EPS) mainly composed of heteropolysaccharides with unique characteristics that make them suitable for biotechnological applications. However, manipulation/optimization of EPS biosynthesis/characteristics is hindered by a poor understanding of the production pathways and the differences between bacterial species. In this work, genes putatively related to different pathways of cyanobacterial EPS polymerization, assembly, and export were targeted for deletion or truncation in the unicellular Synechocystis sp. PCC 6803. No evident phenotypic changes were observed for some mutants in genes occurring in multiple copies in Synechocystis genome, namely wzy (sll0737), wzx (sll5049), kpsM (slr2107), and kpsMwzy (slr2107sll0737), strongly suggesting functional redundancy. In contrast, Deltawzc (Deltasll0923) and Deltawzb (Deltaslr0328) influenced both the amount and composition of the EPS, establishing that Wzc participates in the production of capsular (CPS) and released (RPS) polysaccharides, and Wzb affects RPS production. The structure of Wzb was solved (2.28 A), revealing structural differences relative to other phosphatases involved in EPS production and suggesting a different substrate recognition mechanism. In addition, Wzc showed the ATPase and autokinase activities typical of bacterial tyrosine kinases. Most importantly, Wzb was able to dephosphorylate Wzc in vitro, suggesting that tyrosine phosphorylation/dephosphorylation plays a role in cyanobacterial EPS production.

The role of the tyrosine kinase Wzc (Sll0923) and the phosphatase Wzb (Slr0328) in the production of extracellular polymeric substances (EPS) by Synechocystis PCC 6803.,Pereira SB, Santos M, Leite JP, Flores C, Eisfeld C, Buttel Z, Mota R, Rossi F, De Philippis R, Gales L, Morais-Cabral JH, Tamagnini P Microbiologyopen. 2019 Jan 23:e753. doi: 10.1002/mbo3.753. PMID:30675753^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Pereira SB, Santos M, Leite JP, Flores C, Eisfeld C, Buttel Z, Mota R, Rossi F, De Philippis R, Gales L, Morais-Cabral JH, Tamagnini P. The role of the tyrosine kinase Wzc (Sll0923) and the phosphatase Wzb (Slr0328) in the production of extracellular polymeric substances (EPS) by Synechocystis PCC 6803. Microbiologyopen. 2019 Jan 23:e753. doi: 10.1002/mbo3.753. PMID:30675753 doi:http://dx.doi.org/10.1002/mbo3.753