5twk
From Proteopedia
Crystal Structure of RlmH in Complex with Sinefungin
Structural highlights
FunctionRLMH_ECOLI Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA. Specific for fully assembled 70S ribosomes.[1] [2] Publication Abstract from PubMedEubacterial ribosomal large-subunit methyltransferase H (RlmH) methylates 23S ribosomal RNA pseudouridine 1915 (Psi1915), which lies near the ribosomal decoding center. The smallest member of the SPOUT superfamily of methyltransferases, RlmH lacks the RNA recognition domain found in larger methyltransferases. The catalytic mechanism of RlmH enzyme is unknown. Here, we describe the structures of RlmH bound to S-adenosyl-methionine (SAM) and the methyltransferase inhibitor sinefungin. Our structural and biochemical studies reveal catalytically essential residues in the dimer-mediated asymmetrical active site. One monomer provides the SAM-binding site, whereas the conserved C-terminal tail of the second monomer provides residues essential for catalysis. Our findings elucidate the mechanism by which a small protein dimer assembles a functionally asymmetric architecture. Small methyltransferase RlmH assembles a composite active site to methylate a ribosomal pseudouridine.,Koh CS, Madireddy R, Beane TJ, Zamore PD, Korostelev AA Sci Rep. 2017 Apr 20;7(1):969. doi: 10.1038/s41598-017-01186-5. PMID:28428565[3] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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