5zuu

Structural highlights

Function

CPSF_ARATH Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation. May interact with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition (By similarity). Mediates poly(A) site selection (PubMed:23136375). Binds RNA in a calcium-dependent manner (PubMed:16500995, PubMed:17576667, PubMed:20214900). Exhibits endonuclease activity with an ability to nick and degrade linear as well as circular single-stranded RNA that leaves RNA 3' ends with hydroxyl groups, thus mediating processing of the pre-mRNA as a prelude to the polyadenylation (PubMed:17576667). Involved in the post-transcriptional control, probably via poly(A) addition, of the responses of plants to stress, especially genes mediating tolerance to oxidative stress (PubMed:18545667). Plays a role in the regulation of salicylic acid (SA) production via the control of messenger RNA 3' end processing, thus being a key component of programmed cell death and plant immune responses required for resistance to virulent Pseudomonas syringae pv tomato DC3000 (Pst) (PubMed:24706550).[UniProtKB:O95639]^{[1] [2] [3] [4] [5] [6]}

References

1. ↑ Delaney KJ, Xu R, Zhang J, Li QQ, Yun KY, Falcone DL, Hunt AG. Calmodulin interacts with and regulates the RNA-binding activity of an Arabidopsis polyadenylation factor subunit. Plant Physiol. 2006 Apr;140(4):1507-21. doi: 10.1104/pp.105.070672. Epub 2006 Feb, 24. PMID:16500995 doi:http://dx.doi.org/10.1104/pp.105.070672
2. ↑ Addepalli B, Hunt AG. A novel endonuclease activity associated with the Arabidopsis ortholog of the 30-kDa subunit of cleavage and polyadenylation specificity factor. Nucleic Acids Res. 2007;35(13):4453-63. doi: 10.1093/nar/gkm457. Epub 2007 Jun, 18. PMID:17576667 doi:http://dx.doi.org/10.1093/nar/gkm457
3. ↑ Zhang J, Addepalli B, Yun KY, Hunt AG, Xu R, Rao S, Li QQ, Falcone DL. A polyadenylation factor subunit implicated in regulating oxidative signaling in Arabidopsis thaliana. PLoS One. 2008 Jun 11;3(6):e2410. doi: 10.1371/journal.pone.0002410. PMID:18545667 doi:http://dx.doi.org/10.1371/journal.pone.0002410
4. ↑ Bell SA, Hunt AG. The Arabidopsis ortholog of the 77 kDa subunit of the cleavage stimulatory factor (AtCstF-77) involved in mRNA polyadenylation is an RNA-binding protein. FEBS Lett. 2010 Apr 16;584(8):1449-54. doi: 10.1016/j.febslet.2010.03.007. Epub, 2010 Mar 6. PMID:20214900 doi:http://dx.doi.org/10.1016/j.febslet.2010.03.007
5. ↑ Thomas PE, Wu X, Liu M, Gaffney B, Ji G, Li QQ, Hunt AG. Genome-wide control of polyadenylation site choice by CPSF30 in Arabidopsis. Plant Cell. 2012 Nov;24(11):4376-88. doi: 10.1105/tpc.112.096107. Epub 2012 Nov, 6. PMID:23136375 doi:http://dx.doi.org/10.1105/tpc.112.096107
6. ↑ Bruggeman Q, Garmier M, de Bont L, Soubigou-Taconnat L, Mazubert C, Benhamed M, Raynaud C, Bergounioux C, Delarue M. The Polyadenylation Factor Subunit CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR30: A Key Factor of Programmed Cell Death and a Regulator of Immunity in Arabidopsis. Plant Physiol. 2014 Jun;165(2):732-746. doi: 10.1104/pp.114.236083. Epub 2014 Apr, 4. PMID:24706550 doi:http://dx.doi.org/10.1104/pp.114.236083