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Publication Abstract from PubMed

G-rich sequence found in the regulatory region of RANKL gene associated with homeostasis of bone metabolism folds into a two-quartet basket-type G-quadruplex stabilized by A*G*A and G*G*G base-triads. Perusal of local structural features together with G/A-to-T modifications uncovered critical role of A5 for formation of distinct antiparallel two-quartet and not three-quartet topology that would be expected based on sequence with four GGG-tracts alone. Structural changes induced by A5-to-T5 modification include a switch in orientation and relative positions of G-strands that together with anti to syn reorientation of G12 provide insights into complexity of intricate interactions that influence folding process of G-rich DNA. Understanding the impact of loop residues for overall stability and formation of G-quadruplexes advances our knowledge and ability to predict structures adopted by G-rich sequences which are involved in regulatory mechanisms in cell and may also facilitate drug design driven by structural insights.

Adenine-driven structural switch from two- to three-quartet DNA G-quadruplex.,Plavec J, Lenarcic Zivkovic M, Rozman J Angew Chem Int Ed Engl. 2018 Sep 17. doi: 10.1002/anie.201809328. PMID:30222243^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.