| Structural highlights
Function
RT106_YEAST Histones H3 and H4 chaperone involved in the nucleosome formation and heterochromatin silencing. Required for the deposition of H3K56ac-carrying H3-H4 complex onto newly-replicated DNA. Plays a role in the transcriptional regulation of the cell-cycle dependent histone genes by directly recruiting the SWI/SNF and RSC chromatin remodeling complexes to the histone genes in a cell cycle dependent manner. In cooperation with HIR and ASF1, creates a repressive structure at the core histone gene promoter and contributes to their repression outside of S phase. Involved in regulation of Ty1 transposition.[1] [2] [3] [4] [5] [6] [7] [8] [9] [10] [11] [12]
Publication Abstract from PubMed
Biogenesis of eukaryotic box C/D small nucleolar ribonucleoproteins initiates co-transcriptionally and requires the action of the assembly machinery including the Hsp90/R2TP complex, the Rsa1p:Hit1p heterodimer and the Bcd1 protein. We present genetic interactions between the Rsa1p-encoding gene and genes involved in chromatin organization including RTT106 that codes for the H3-H4 histone chaperone Rtt106p controlling H3K56ac deposition. We show that Bcd1p binds Rtt106p and controls its transcription-dependent recruitment by reducing its association with RNA polymerase II, modulating H3K56ac levels at gene body. We reveal the 3D structures of the free and Rtt106p-bound forms of Bcd1p using nuclear magnetic resonance and X-ray crystallography. The interaction is also studied by a combination of biophysical and proteomic techniques. Bcd1p interacts with a region that is distinct from the interaction interface between the histone chaperone and histone H3. Our results are evidence for a protein interaction interface for Rtt106p that controls its transcription-associated activity.
The box C/D snoRNP assembly factor Bcd1 interacts with the histone chaperone Rtt106 and controls its transcription dependent activity.,Bragantini B, Charron C, Bourguet M, Paul A, Tiotiu D, Rothe B, Marty H, Terral G, Hessmann S, Decourty L, Chagot ME, Strub JM, Massenet S, Bertrand E, Quinternet M, Saveanu C, Cianferani S, Labialle S, Manival X, Charpentier B Nat Commun. 2021 Mar 25;12(1):1859. doi: 10.1038/s41467-021-22077-4. PMID:33767140[13]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
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- ↑ Huang S, Zhou H, Katzmann D, Hochstrasser M, Atanasova E, Zhang Z. Rtt106p is a histone chaperone involved in heterochromatin-mediated silencing. Proc Natl Acad Sci U S A. 2005 Sep 20;102(38):13410-5. Epub 2005 Sep 12. PMID:16157874 doi:http://dx.doi.org/0506176102
- ↑ Huang S, Zhou H, Tarara J, Zhang Z. A novel role for histone chaperones CAF-1 and Rtt106p in heterochromatin silencing. EMBO J. 2007 May 2;26(9):2274-83. Epub 2007 Apr 5. PMID:17410207 doi:http://dx.doi.org/7601670
- ↑ Fillingham J, Kainth P, Lambert JP, van Bakel H, Tsui K, Pena-Castillo L, Nislow C, Figeys D, Hughes TR, Greenblatt J, Andrews BJ. Two-color cell array screen reveals interdependent roles for histone chaperones and a chromatin boundary regulator in histone gene repression. Mol Cell. 2009 Aug 14;35(3):340-51. PMID:19683497 doi:http://dx.doi.org/S1097-2765(09)00461-4
- ↑ Burgess RJ, Zhou H, Han J, Zhang Z. A role for Gcn5 in replication-coupled nucleosome assembly. Mol Cell. 2010 Feb 26;37(4):469-80. doi: 10.1016/j.molcel.2010.01.020. PMID:20188666 doi:http://dx.doi.org/10.1016/j.molcel.2010.01.020
- ↑ Wan Y, Chen W, Xing J, Tan J, Li B, Chen H, Lin Z, Chiang JH, Saleem RA. Transcriptome profiling reveals a novel role for trichostatin A in antagonizing histone chaperone Chz1 mediated telomere anti-silencing. FEBS Lett. 2011 Aug 4;585(15):2519-25. doi: 10.1016/j.febslet.2011.06.036. Epub, 2011 Jul 14. PMID:21763693 doi:http://dx.doi.org/10.1016/j.febslet.2011.06.036
- ↑ Kurat CF, Lambert JP, van Dyk D, Tsui K, van Bakel H, Kaluarachchi S, Friesen H, Kainth P, Nislow C, Figeys D, Fillingham J, Andrews BJ. Restriction of histone gene transcription to S phase by phosphorylation of a chromatin boundary protein. Genes Dev. 2011 Dec 1;25(23):2489-501. doi: 10.1101/gad.173427.111. PMID:22156209 doi:http://dx.doi.org/10.1101/gad.173427.111
- ↑ Yu Y, Srinivasan M, Nakanishi S, Leatherwood J, Shilatifard A, Sternglanz R. A conserved patch near the C terminus of histone H4 is required for genome stability in budding yeast. Mol Cell Biol. 2011 Jun;31(11):2311-25. doi: 10.1128/MCB.01432-10. Epub 2011 Mar , 28. PMID:21444721 doi:http://dx.doi.org/10.1128/MCB.01432-10
- ↑ Ferreira ME, Flaherty K, Prochasson P. The Saccharomyces cerevisiae histone chaperone Rtt106 mediates the cell cycle recruitment of SWI/SNF and RSC to the HIR-dependent histone genes. PLoS One. 2011;6(6):e21113. doi: 10.1371/journal.pone.0021113. Epub 2011 Jun 15. PMID:21698254 doi:http://dx.doi.org/10.1371/journal.pone.0021113
- ↑ Amin AD, Dimova DK, Ferreira ME, Vishnoi N, Hancock LC, Osley MA, Prochasson P. The mitotic Clb cyclins are required to alleviate HIR-mediated repression of the yeast histone genes at the G1/S transition. Biochim Biophys Acta. 2012 Jan;1819(1):16-27. doi: 10.1016/j.bbagrm.2011.09.003. , Epub 2011 Sep 28. PMID:21978826 doi:http://dx.doi.org/10.1016/j.bbagrm.2011.09.003
- ↑ Silva AC, Xu X, Kim HS, Fillingham J, Kislinger T, Mennella TA, Keogh MC. The replication-independent histone H3-H4 chaperones HIR, ASF1, and RTT106 co-operate to maintain promoter fidelity. J Biol Chem. 2012 Jan 13;287(3):1709-18. doi: 10.1074/jbc.M111.316489. Epub 2011 , Nov 29. PMID:22128187 doi:http://dx.doi.org/10.1074/jbc.M111.316489
- ↑ Liu Y, Huang H, Zhou BO, Wang SS, Hu Y, Li X, Liu J, Zang J, Niu L, Wu J, Zhou JQ, Teng M, Shi Y. Structural analysis of Rtt106p reveals a DNA binding role required for heterochromatin silencing. J Biol Chem. 2010 Feb 5;285(6):4251-62. Epub 2009 Dec 10. PMID:20007951 doi:10.1074/jbc.M109.055996
- ↑ Bragantini B, Charron C, Bourguet M, Paul A, Tiotiu D, Rothe B, Marty H, Terral G, Hessmann S, Decourty L, Chagot ME, Strub JM, Massenet S, Bertrand E, Quinternet M, Saveanu C, Cianferani S, Labialle S, Manival X, Charpentier B. The box C/D snoRNP assembly factor Bcd1 interacts with the histone chaperone Rtt106 and controls its transcription dependent activity. Nat Commun. 2021 Mar 25;12(1):1859. doi: 10.1038/s41467-021-22077-4. PMID:33767140 doi:http://dx.doi.org/10.1038/s41467-021-22077-4
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