| Structural highlights
Function
MSP1_PLAF7 During the asexual blood stage, involved in merozoite egress from host erythrocytes possibly via its interaction with the host cytoskeleton protein spectrin resulting in the destabilization of the host cytoskeleton and thus leading to erythrocyte cell membrane rupture (PubMed:26468747). Involved in the binding to host erythrocytes and is required for host erythrocyte invasion (PubMed:12692305, PubMed:21175202, PubMed:25778531, PubMed:36202833).[1] [2] [3] [4] [5] By binding to host proinflammatory cytokine S100P may interfere with host immune responses.[6] Involved in merozoite invasion of host erythrocytes (PubMed:1694225, PubMed:29511044). May play a role in the biogenesis and/or function of the food vacuole during the intraerythrocytic development (Probable).[7] [8] [9]
Publication Abstract from PubMed
The merozoite surface protein 1 (MSP-1) is the most abundant protein on the surface of the erythrocyte-invading Plasmodium merozoite, the causative agent of malaria. MSP-1 is essential for merozoite formation, entry into and escape from erythrocytes, and is a promising vaccine candidate. Here, we present monomeric and dimeric structures of full-length MSP-1. MSP-1 adopts an unusual fold with a large central cavity. Its fold includes several coiled-coils and shows structural homology to proteins associated with membrane and cytoskeleton interactions. MSP-1 formed dimers through these domains in a concentration-dependent manner. Dimerization is affected by the presence of the erythrocyte cytoskeleton protein spectrin, which may compete for the dimerization interface. Our work provides structural insights into the possible mode of interaction of MSP-1 with erythrocytes and establishes a framework for future investigations into the role of MSP-1 in Plasmodium infection and immunity.
Structure of the merozoite surface protein 1 from Plasmodium falciparum.,Dijkman PM, Marzluf T, Zhang Y, Chang SS, Helm D, Lanzer M, Bujard H, Kudryashev M Sci Adv. 2021 Jun 2;7(23):eabg0465. doi: 10.1126/sciadv.abg0465. Print 2021 Jun. PMID:34078606[10]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Goel VK, Li X, Chen H, Liu SC, Chishti AH, Oh SS. Band 3 is a host receptor binding merozoite surface protein 1 during the Plasmodium falciparum invasion of erythrocytes. Proc Natl Acad Sci U S A. 2003 Apr 29;100(9):5164-9. PMID:12692305 doi:10.1073/pnas.0834959100
- ↑ Ranjan R, Chugh M, Kumar S, Singh S, Kanodia S, Hossain MJ, Korde R, Grover A, Dhawan S, Chauhan VS, Reddy VS, Mohmmed A, Malhotra P. Proteome analysis reveals a large merozoite surface protein-1 associated complex on the Plasmodium falciparum merozoite surface. J Proteome Res. 2011 Feb 4;10(2):680-91. PMID:21175202 doi:10.1021/pr100875y
- ↑ Baldwin MR, Li X, Hanada T, Liu SC, Chishti AH. Merozoite surface protein 1 recognition of host glycophorin A mediates malaria parasite invasion of red blood cells. Blood. 2015 Apr 23;125(17):2704-11. PMID:25778531 doi:10.1182/blood-2014-11-611707
- ↑ Das S, Hertrich N, Perrin AJ, Withers-Martinez C, Collins CR, Jones ML, Watermeyer JM, Fobes ET, Martin SR, Saibil HR, Wright GJ, Treeck M, Epp C, Blackman MJ. Processing of Plasmodium falciparum Merozoite Surface Protein MSP1 Activates a Spectrin-Binding Function Enabling Parasite Egress from RBCs. Cell Host Microbe. 2015 Oct 14;18(4):433-44. PMID:26468747 doi:10.1016/j.chom.2015.09.007
- ↑ Patel PN, Dickey TH, Hopp CS, Diouf A, Tang WK, Long CA, Miura K, Crompton PD, Tolia NH. Neutralizing and interfering human antibodies define the structural and mechanistic basis for antigenic diversion. Nat Commun. 2022 Oct 6;13(1):5888. doi: 10.1038/s41467-022-33336-3. PMID:36202833 doi:http://dx.doi.org/10.1038/s41467-022-33336-3
- ↑ Waisberg M, Cerqueira GC, Yager SB, Francischetti IM, Lu J, Gera N, Srinivasan P, Miura K, Rada B, Lukszo J, Barbian KD, Leto TL, Porcella SF, Narum DL, El-Sayed N, Miller LH, Pierce SK. Plasmodium falciparum merozoite surface protein 1 blocks the proinflammatory protein S100P. Proc Natl Acad Sci U S A. 2012 Apr 3;109(14):5429-34. PMID:22431641 doi:10.1073/pnas.1202689109
- ↑ Blackman MJ, Heidrich HG, Donachie S, McBride JS, Holder AA. A single fragment of a malaria merozoite surface protein remains on the parasite during red cell invasion and is the target of invasion-inhibiting antibodies. J Exp Med. 1990 Jul 1;172(1):379-82. PMID:1694225 doi:10.1084/jem.172.1.379
- ↑ Paul G, Deshmukh A, Kumar Chourasia B, Kalamuddin M, Panda A, Kumar Singh S, Gupta PK, Mohmmed A, Chauhan VS, Theisen M, Malhotra P. Protein-protein interaction studies reveal the Plasmodium falciparum merozoite surface protein-1 region involved in a complex formation that binds to human erythrocytes. Biochem J. 2018 Mar 29;475(6):1197-1209. PMID:29511044 doi:10.1042/BCJ20180017
- ↑ Dluzewski AR, Ling IT, Hopkins JM, Grainger M, Margos G, Mitchell GH, Holder AA, Bannister LH. Formation of the food vacuole in Plasmodium falciparum: a potential role for the 19 kDa fragment of merozoite surface protein 1 (MSP1(19)). PLoS One. 2008 Aug 29;3(8):e3085. PMID:18769730 doi:10.1371/journal.pone.0003085
- ↑ Dijkman PM, Marzluf T, Zhang Y, Chang SS, Helm D, Lanzer M, Bujard H, Kudryashev M. Structure of the merozoite surface protein 1 from Plasmodium falciparum. Sci Adv. 2021 Jun 2;7(23):eabg0465. PMID:34078606 doi:10.1126/sciadv.abg0465
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