7cbe

Structural highlights

Function

METAS_ECOLI Transfers a succinyl group from succinyl-CoA to L-homoserine, forming succinyl-L-homoserine (PubMed:10572016, PubMed:17442255, PubMed:17302437, PubMed:28581482). Utilizes a ping-pong kinetic mechanism in which the succinyl group of succinyl-CoA is initially transferred to the enzyme to form a succinyl-enzyme intermediate before subsequent transfer to homoserine to form the final product, O-succinylhomoserine (PubMed:10572016, PubMed:17442255, PubMed:17302437). Cannot use acetyl-CoA (PubMed:10572016).^{[1] [2] [3] [4]}

References

1. ↑ Born TL, Blanchard JS. Enzyme-catalyzed acylation of homoserine: mechanistic characterization of the Escherichia coli metA-encoded homoserine transsuccinylase. Biochemistry. 1999 Oct 26;38(43):14416-23. doi: 10.1021/bi991710o. PMID:10572016 doi:http://dx.doi.org/10.1021/bi991710o
2. ↑ Ziegler K, Noble SM, Mutumanje E, Bishop B, Huddler DP, Born TL. Identification of catalytic cysteine, histidine, and lysine residues in Escherichia coli homoserine transsuccinylase. Biochemistry. 2007 Mar 13;46(10):2674-83. doi: 10.1021/bi0620252. Epub 2007 Feb, 16. PMID:17302437 doi:http://dx.doi.org/10.1021/bi0620252
3. ↑ Coe DM, Viola RE. Assessing the roles of essential functional groups in the mechanism of homoserine succinyltransferase. Arch Biochem Biophys. 2007 May 15;461(2):211-8. doi: 10.1016/j.abb.2007.03.004., Epub 2007 Apr 2. PMID:17442255 doi:http://dx.doi.org/10.1016/j.abb.2007.03.004
4. ↑ Bastard K, Perret A, Mariage A, Bessonnet T, Pinet-Turpault A, Petit JL, Darii E, Bazire P, Vergne-Vaxelaire C, Brewee C, Debard A, Pellouin V, Besnard-Gonnet M, Artiguenave F, Medigue C, Vallenet D, Danchin A, Zaparucha A, Weissenbach J, Salanoubat M, de Berardinis V. Parallel evolution of non-homologous isofunctional enzymes in methionine biosynthesis. Nat Chem Biol. 2017 Aug;13(8):858-866. doi: 10.1038/nchembio.2397. Epub 2017 Jun , 5. PMID:28581482 doi:http://dx.doi.org/10.1038/nchembio.2397