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Publication Abstract from PubMed

Photosynthetic reaction centers (RCs) from Rhodobacter sphaeroides were engineered to vary the electronic properties of a key tyrosine (M210) close to an essential electron transfer component via its replacement with site-specific, genetically encoded noncanonical amino acid tyrosine analogs. High fidelity of noncanonical amino acid incorporation was verified with mass spectrometry and X-ray crystallography and demonstrated that RC variants exhibit no significant structural alterations relative to wild type (WT). Ultrafast transient absorption spectroscopy indicates the excited primary electron donor, P*, decays via a approximately 4-ps and a approximately 20-ps population to produce the charge-separated state P(+)H(A)(-) in all variants. Global analysis indicates that in the approximately 4-ps population, P(+)H(A)(-) forms through a two-step process, P*--> P(+)B(A)(-)--> P(+)H(A)(-), while in the approximately 20-ps population, it forms via a one-step P* --> P(+)H(A)(-) superexchange mechanism. The percentage of the P* population that decays via the superexchange route varies from approximately 25 to approximately 45% among variants, while in WT, this percentage is approximately 15%. Increases in the P* population that decays via superexchange correlate with increases in the free energy of the P(+)B(A)(-) intermediate caused by a given M210 tyrosine analog. This was experimentally estimated through resonance Stark spectroscopy, redox titrations, and near-infrared absorption measurements. As the most energetically perturbative variant, 3-nitrotyrosine at M210 creates an approximately 110-meV increase in the free energy of P(+)B(A)(-) along with a dramatic diminution of the 1,030-nm transient absorption band indicative of P(+)B(A)(-) formation. Collectively, this work indicates the tyrosine at M210 tunes the mechanism of primary electron transfer in the RC.

Photosynthetic reaction center variants made via genetic code expansion show Tyr at M210 tunes the initial electron transfer mechanism.,Weaver JB, Lin CY, Faries KM, Mathews II, Russi S, Holten D, Kirmaier C, Boxer SG Proc Natl Acad Sci U S A. 2021 Dec 21;118(51):e2116439118. doi: , 10.1073/pnas.2116439118. PMID:34907018^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.