7q86

From Proteopedia

Crystal structure of human peroxisomal acyl-Co-A oxidase 1a, FAD-bound-form

Structural highlights

7q86 is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.09Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

A0A024R8L7_HUMAN

Publication Abstract from PubMed

Peroxisomal acyl-CoA oxidase 1a (ACOX1a) catalyzes the first and rate-limiting step of fatty acid oxidation, the conversion of acyl-CoAs to 2-trans-enoyl-CoAs. The dysfunction of human ACOX1a (hACOX1a) leads to deterioration of the nervous system manifesting in myeloneuropathy, hypotonia and convulsions. Crystal structures of hACOX1a in apo- and cofactor (FAD)-bound forms were solved at 2.00 and 2.09 A resolution, respectively. hACOX1a exists as a homo-dimer with solvation free energy gain (DeltaG(o)) of -44.7 kcal mol(-1). Two FAD molecules bind at the interface of protein monomers completing the active sites. The substrate binding cleft of hACOX1a is wider compared to human mitochondrial very-long chain specific acyl-CoA dehydrogenase. Mutations (p.G178C, p.M278V and p.N237S) reported to cause dysfunctionality of hACOX1a are analyzed on its 3D-structure to understand structure-function related perturbations and explain the associated phenotypes.

Crystal structures of apo- and FAD-bound human peroxisomal acyl-CoA oxidase provide mechanistic basis explaining clinical observations.,Sonani RR, Blat A, Dubin G Int J Biol Macromol. 2022 Feb 8;205:203-210. doi: 10.1016/j.ijbiomac.2022.02.008. PMID:35149097^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Sonani RR, Blat A, Dubin G. Crystal structures of apo provide mechanistic basis explaining clinical observations. Int J Biol Macromol. 2022 Apr 30;205:203-210. PMID:35149097 doi:10.1016/j.ijbiomac.2022.02.008