8gu0

Publication Abstract from PubMed

RadH is one of the flavin-dependent halogenases that has previously exhibited promising catalytic activity towards hydroxycoumarin, hydroxyisoquinoline, and phenolic derivatives. Here, we evaluated new functional homologs of RadH and expanded its specificities for the halogenation of non-tryptophan-derived, heterocyclic scaffolds. Our investigation revealed that RadH could effectively halogenate hydroxyquinoline and hydroxybenzothiophene. Assay optimization studies revealed the need to balance the various co-factor concentrations and where a GDHi co-factor recycling system most significantly improves the conversion and efficiency of the reaction. A crystal structure of RadH was also obtained with a resolution of 2.4 A, and docking studies were conducted to pinpoint the binding and catalytic sites for substrates.

Further Characterization of Fungal Halogenase RadH and Its Homologs.,Peh G, Gunawan GA, Tay T, Tiong E, Tan LL, Jiang S, Goh YL, Ye S, Wong J, Brown CJ, Zhao H, Ang EL, Wong FT, Lim YH Biomolecules. 2023 Jul 6;13(7):1081. doi: 10.3390/biom13071081. PMID:37509117^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.