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Publication Abstract from PubMed

Methylorubrum extorquens, a facultative methylotroph, assimilates C(1) compounds and accumulates poly-beta-hydroxylbutyrate (PHB) as carbon and energy sources. The ethylmalonyl pathway is central to the carbon metabolism of M. extorquens, and is linked with a serine cycle and a PHB biosynthesis pathway. Understanding the ethylmalonyl pathway is vital in utilizing methylotrophs to produce value-added chemicals. In this study, we determined the crystal structure of the mesaconyl-CoA hydratase from M. extorquens (MeMeaC) that catalyzes the reversible conversion of mesaconyl-CoA to beta-methylmalyl-CoA. The crystal structure of MeMeaC revealed that the enzyme belongs to the MaoC-like dehydratase domain superfamily and functions as a trimer. In our current MeMeaC structure, malic acid occupied the substrate binding site, which reveals how MeMeaC recognizes the beta-methylmalyl-moiety of its substrate. The active site of the enzyme was further speculated by comparing its structure with those of other MaoC-like hydratases.

Crystal Structure of Mesaconyl-CoA Hydratase from Methylorubrum extorquens CM4.,Ahn JW, Hong J, Kim KJ J Microbiol Biotechnol. 2023 Apr 28;33(4):485-492. doi: 10.4014/jmb.2212.12003. , Epub 2023 Jan 26. PMID:36788474^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.