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Publication Abstract from PubMed

Monoclonal antibodies (mAbs) that target the P-amyloid peptide (Abeta) are important Alzheimer's disease research tools and are now being used as Alzheimer's disease therapies. Conformation-specific mAbs that target oligomeric and fibrillar Abeta assemblies are of particular interest, as these assemblies are associated with Alzheimer's disease pathogenesis and progression. This paper reports the generation of rabbit mAbs against two different triangular trimers derived from Abeta. These antibodies are the first mAbs generated against Abeta oligomer mimics in which the high-resolution structures of the oligomers are known. We describe the isolation of the mAbs using single B-cell sorting of peripheral blood mononuclear cells (PBMCs) from immunized rabbits, the selectivity of the mAbs for the triangular trimers, the immunoreactivity of the mAbs with aggregated Abeta(42), and the immunoreactivity of the mAbs in brain tissue from the 5xFAD Alzheimer's disease mouse model. The characterization of these mAbs against structurally defined trimers derived from Abeta enhances understanding of antibody-amyloid recognition and may benefit the development of diagnostics and immunotherapies in Alzheimer's disease.

Generation and Study of Antibodies against Two Triangular Trimers Derived from Abeta.,Kreutzer AG, Malonis RJ, Parrocha CMT, Tong K, Guaglianone G, Nguyen JT, Diab MN, Lai JR, Nowick JS Pept Sci (Hoboken). 2024 Mar;116(2):e24333. doi: 10.1002/pep2.24333. Epub 2023 , Sep 18. PMID:38644932^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.