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9g0w

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auxin transporter PIN8 as asymmetric dimer (inward/outward) with 2,4-D bound in the inward vestibule prebinding state

Structural highlights

9g0w is a 2 chain structure with sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 3.54Å
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PIN8_ARATH Component of the intracellular auxin-transport pathway in the male gametophyte (PubMed:20439545, PubMed:22540348). Involved in the regulation of auxin homeostasis in pollen (PubMed:22540348). Involved in the efflux of auxin from the endoplasmic reticulum into the cytoplasm (PubMed:22760640). PIN5 and PIN8 may have an antagonistic/compensatory activity (PubMed:22760640, PubMed:22990451). Involved in the control of vein patterning (PubMed:23437008, PubMed:24304505). Redundantly with PIN6, inhibits the vein-formation-promoting functions of PIN5 (PubMed:26560462). PIN5, PIN6, and PIN8 control vein network geometry, but they are expressed in mutually exclusive domains of leaf vascular cells (PubMed:26560462).^[1] ^[2] ^[3] ^[4] ^[5] ^[6] ^[7]

References

↑ Ganguly A, Lee SH, Cho M, Lee OR, Yoo H, Cho HT. Differential auxin-transporting activities of PIN-FORMED proteins in Arabidopsis root hair cells. Plant Physiol. 2010 Jul;153(3):1046-61. doi: 10.1104/pp.110.156505. Epub 2010 May, 3. PMID:20439545 doi:http://dx.doi.org/10.1104/pp.110.156505
↑ Dal Bosco C, Dovzhenko A, Liu X, Woerner N, Rensch T, Eismann M, Eimer S, Hegermann J, Paponov IA, Ruperti B, Heberle-Bors E, Touraev A, Cohen JD, Palme K. The endoplasmic reticulum localized PIN8 is a pollen-specific auxin carrier involved in intracellular auxin homeostasis. Plant J. 2012 Sep;71(5):860-70. doi: 10.1111/j.1365-313X.2012.05037.x. Epub 2012 , Jul 3. PMID:22540348 doi:http://dx.doi.org/10.1111/j.1365-313X.2012.05037.x
↑ Ding Z, Wang B, Moreno I, Duplakova N, Simon S, Carraro N, Reemmer J, Pencik A, Chen X, Tejos R, Skupa P, Pollmann S, Mravec J, Petrasek J, Zazimalova E, Honys D, Rolcik J, Murphy A, Orellana A, Geisler M, Friml J. ER-localized auxin transporter PIN8 regulates auxin homeostasis and male gametophyte development in Arabidopsis. Nat Commun. 2012 Jul 3;3:941. doi: 10.1038/ncomms1941. PMID:22760640 doi:http://dx.doi.org/10.1038/ncomms1941
↑ Sawchuk MG, Edgar A, Scarpella E. Patterning of leaf vein networks by convergent auxin transport pathways. PLoS Genet. 2013;9(2):e1003294. doi: 10.1371/journal.pgen.1003294. Epub 2013 Feb , 21. PMID:23437008 doi:http://dx.doi.org/10.1371/journal.pgen.1003294
↑ Sawchuk MG, Scarpella E. Control of vein patterning by intracellular auxin transport. Plant Signal Behav. 2013 Nov;8(11):e27205. doi: 10.4161/psb.27205. Epub 2013 Dec , 4. PMID:24304505 doi:http://dx.doi.org/10.4161/psb.27205
↑ Verna C, Sawchuk MG, Linh NM, Scarpella E. Control of vein network topology by auxin transport. BMC Biol. 2015 Nov 11;13:94. doi: 10.1186/s12915-015-0208-3. PMID:26560462 doi:http://dx.doi.org/10.1186/s12915-015-0208-3
↑ Dal Bosco C, Dovzhenko A, Palme K. Intracellular auxin transport in pollen: PIN8, PIN5 and PILS5. Plant Signal Behav. 2012 Nov;7(11):1504-5. doi: 10.4161/psb.21953. Epub 2012 Sep , 18. PMID:22990451 doi:http://dx.doi.org/10.4161/psb.21953