9gyh
From Proteopedia
HEW Lysozyme with His 15 functionalized with iodoacetamide
Structural highlights
FunctionLYSC_CHICK Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.[1] Publication Abstract from PubMedHen egg-white lysozyme (HEWL) is a small polycationic protein which is highly soluble and stable. This has led to it becoming a ;molecular laboratory' where chemical biological operations and structural techniques are tested. To date, HEWL accounts for 1233 PDB entries, roughly 0.5% of the total, making it the best-represented protein in the PDB. With the aim of unambiguously identifying the N atom of the His15 side chain that is most reactive towards iodoacetamide, the structure of chemically modified HEWL was determined by crystallizing it using the ;15 minutes lysozyme' protocol. This protocol invariably yields tetragonal crystals of the unmodified protein. To our surprise, we found that the crystals of the modified protein had similar unit-cell parameters but that refinement was only possible when considering an orthorhombic system. The structure of His15 acetamide-modified hen egg-white lysozyme: a nice surprise from an old friend.,Malanho da Silva J, Lanuza J, Bruno F, Calderone V, Ravera E Acta Crystallogr F Struct Biol Commun. 2025 Feb 1;81(Pt 2):41-46. doi: , 10.1107/S2053230X2500010X. Epub 2025 Jan 13. PMID:39804568[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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