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Publication Abstract from PubMed

In archaea and metazoa, tRNA exons are ligated by the RNA ligases RtcB and RTCB, respectively. The metazoan RTCB forms a stable complex with four additional subunits, DDX1, FAM98B, CGI99 and ASHWIN. The role and assembly of these four components remain elusive. Furthermore, we lack structural information of how RNA substrates are recognized by 3'-5' tRNA ligases. Here, we use thiol-based chemical cross-linking to confirm the involvement of specific residues of RtcB in RNA binding and we present a cryo-electron microscopy structure of the purified five-subunit D. rerio tRNA ligase complex. The structure implies that the DDX1 helicase module is mobile and can modulate the activity of RTCB. Taken together, the presented results enhance our mechanistic understanding of RNA binding by 3'-5' tRNA splicing ligases and architecture of the metazoan tRNA ligase complex.

Structural and biochemical characterization of the 3'-5' tRNA splicing ligases.,Chamera S, Zajko W, Czarnocki-Cieciura M, Jaciuk M, Koziej L, Nowak J, Wycisk K, Sroka M, Chramiec-Glabik A, Smietanski M, Golebiowski F, Warminski M, Jemielity J, Glatt S, Nowotny M J Biol Chem. 2025 Apr 10:108506. doi: 10.1016/j.jbc.2025.108506. PMID:40220997^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.