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1l8f

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Structure of 20K-endoglucanase from Melanocarpus albomyces at 1.8 A

Structural highlights

1l8f is a 1 chain structure with sequence from Melanocarpus albomyces. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.8Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q8J0K8_MELAO

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The crystal structure of the 20K endoglucanase from the thermophilic fungus Melanocarpus albomyces (Ma20k) has been determined. The structure was refined to 1.8 A resolution using data obtained at 120 K. Ma20k belongs to glycoside hydrolase family 45. The three-dimensional structures of endoglucanase V (EGV) from the fungus Humicola insolens and of an endoglucanase from H. grisea var. thermoidea have previously been determined. The overall structure of Ma20k consists of a six-stranded beta-barrel domain similar to that found previously in family 45 endoglucanases. The flexible loop between strands V and VI, which was disordered in the uncomplexed structures of the Humicola endoglucanases but was ordered in complexed structures of EGV, is found to be well ordered in the native structure of Ma20k. The structure of Ma20k allows comparison between thermophilic and mesophilic proteins of family 45 and different principles for thermostability are discussed.

Structure of 20K endoglucanase from Melanocarpus albomyces at 1.8 A resolution.,Valjakka J, Rouvinen J Acta Crystallogr D Biol Crystallogr. 2003 Apr;59(Pt 4):765-8. Epub 2003, Mar 25. PMID:12657806^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Valjakka J, Rouvinen J. Structure of 20K endoglucanase from Melanocarpus albomyces at 1.8 A resolution. Acta Crystallogr D Biol Crystallogr. 2003 Apr;59(Pt 4):765-8. Epub 2003, Mar 25. PMID:12657806