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3ajf

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Structural insigths into dsRNA binding and RNA silencing suppression by NS3 protein of rice hoja blanca tenuivirus

Structural highlights

3ajf is a 4 chain structure with sequence from Rice hoja blanca virus - cr. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

VSR_RHBVC Acts as a suppressor of RNA-mediated gene silencing, also known as post-transcriptional gene silencing (PTGS), presumably through the binding of dsRNA (By similarity).

Publication Abstract from PubMed

Rice Hoja Blanca Tenuivirus (RHBV), a negative strand RNA virus, has been identified to infect rice and is widely transmitted by the insect vector. NS3 protein encoded by RHBV RNA3 was reported to be a potent RNAi suppressor to counterdefense RNA silencing in plants, insect cells, and mammalian cells. Here, we report the crystal structure of the N-terminal domain of RHBV NS3 (residues 21-114) at 2.0 A. RHBV NS3 N-terminal domain forms a dimer by two pairs of alpha-helices in an anti-parallel mode, with one surface harboring a shallow groove at the dimension of 20 A x 30 A for putative dsRNA binding. In vitro RNA binding assay and RNA silencing suppression assay have demonstrated that the structural conserved residues located along this shallow groove, such as Arg50, His51, Lys77, and His85, participate in dsRNA binding and RNA silencing suppression. Our results provide the initial structural implications in understanding the RNAi suppression mechanism by RHBV NS3.

Structural implications into dsRNA binding and RNA silencing suppression by NS3 protein of Rice Hoja Blanca Tenuivirus.,Yang X, Tan SH, Teh YJ, Yuan YA RNA. 2011 May;17(5):903-11. Epub 2011 Apr 1. PMID:21460234^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Yang X, Tan SH, Teh YJ, Yuan YA. Structural implications into dsRNA binding and RNA silencing suppression by NS3 protein of Rice Hoja Blanca Tenuivirus. RNA. 2011 May;17(5):903-11. Epub 2011 Apr 1. PMID:21460234 doi:10.1261/rna.2552811