3nbc

Structural highlights

Function

CNL_CLINE Lectin specific for terminal, non-reducing N-acetylgalactosamine (Gal-NAc)-containing carbohydrates including N,N'-diacetyllactosediamine/LDN (GalNAcbeta1-4GlcNAc, LacdiNAc). Specific also for carbohydrates containing N-acetylglucosamine (-GlcNAc) or N-acetyllactosamine (-Galbeta1-4GlcNAc) at the reducing end. Agglutinates human blood group A, AB, B and O erythrocytes with a strong preference for group A. Agglutinates bovine erythrocytes with a very low specificity (PubMed:19100814, PubMed:22298779). Binds carbohydrates bivalently, which is required for its biological activity (PubMed:22298779). Exhibits insecticidal activity against the fruit fly D.melanogaster, mosquito A.aegypti, and amoebozoa A.castellanii. Has anti-nutritional activity against Colorado potato beetle L.decemlineata, and against worm C.elegans (PubMed:21556921, PubMed:21486374). Has antiproliferative activity against human leukemic T-cells (PubMed:19100814). Has an immunostimulatory effect on human antigen-presenting dendritic cells, which are subsequently able to induce efficient T-cell immune responses (PubMed:22044067).^{[1] [2] [3] [4] [5]}

See Also

• Ricin 3D structures

References

1. ↑ Pohleven J, Obermajer N, Sabotic J, Anzlovar S, Sepcić K, Kos J, Kralj B, Strukelj B, Brzin J. Purification, characterization and cloning of a ricin B-like lectin from mushroom Clitocybe nebularis with antiproliferative activity against human leukemic T cells. Biochim Biophys Acta. 2009 Mar;1790(3):173-81. PMID:19100814 doi:10.1016/j.bbagen.2008.11.006
2. ↑ Bleuler-Martínez S, Butschi A, Garbani M, Wälti MA, Wohlschlager T, Potthoff E, Sabotiĉ J, Pohleven J, Lüthy P, Hengartner MO, Aebi M, Künzler M. A lectin-mediated resistance of higher fungi against predators and parasites. Mol Ecol. 2011 Jul;20(14):3056-70. PMID:21486374 doi:10.1111/j.1365-294X.2011.05093.x
3. ↑ Pohleven J, Brzin J, Vrabec L, Leonardi A, Cokl A, Strukelj B, Kos J, Sabotič J. Basidiomycete Clitocybe nebularis is rich in lectins with insecticidal activities. Appl Microbiol Biotechnol. 2011 Aug;91(4):1141-8. PMID:21556921 doi:10.1007/s00253-011-3236-0
4. ↑ Svajger U, Pohleven J, Kos J, Strukelj B, Jeras M. CNL, a ricin B-like lectin from mushroom Clitocybe nebularis, induces maturation and activation of dendritic cells via the toll-like receptor 4 pathway. Immunology. 2011 Dec;134(4):409-18. PMID:22044067 doi:10.1111/j.1365-2567.2011.03500.x
5. ↑ Pohleven J, Renko M, Magister S, Smith DF, Kuenzler M, Strukelj B, Turk D, Kos J, Sabotic J. Bivalent carbohydrate binding is required for biological activity of CNL, the LacdiNAc (GalNAcbeta1-4GlcNAc)-specific lectin from basidiomycete Clitocybe nebularis. J Biol Chem. 2012 Feb 1. PMID:22298779 doi:10.1074/jbc.M111.317263