6rj0
From Proteopedia
Atlantic Cod Nervous Necrosis Virus-Like Particle produced in Nicotiana benthamiana
Structural highlights
FunctionCAPSD_SJNNV Capsid protein alpha self-assembles to form an icosahedral procapsid with a T=3 symmetry, about 30 nm in diameter, and consisting of 60 capsid proteins trimers. The capsid encapsulates the two genomic RNAs (By similarity). Publication Abstract from PubMedVirus-like particles (VLPs) of the fish virus, Atlantic Cod Nervous necrosis virus (ACNNV), were successfully produced by transient expression of the coat protein in Nicotiana benthamiana plants. VLPs could also be produced in transgenic tobacco BY-2 cells. The protein extracted from plants self-assembled into T = 3 particles, that appeared to be morphologically similar to previously analyzed NNV VLPs when analyzed by high resolution cryo-electron microscopy. Administration of the plant-produced VLPs to sea bass (Dicentrarchus labrax) showed that they could protect the fish against subsequent virus challenge, indicating that plant-produced vaccines may have a substantial future role in aquaculture. Plant-Made Nervous Necrosis Virus-Like Particles Protect Fish Against Disease.,Marsian J, Hurdiss DL, Ranson NA, Ritala A, Paley R, Cano I, Lomonossoff GP Front Plant Sci. 2019 Jul 9;10:880. doi: 10.3389/fpls.2019.00880. eCollection, 2019. PMID:31354759[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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