7v6h

Publication Abstract from PubMed

The catalog of enzymes known to catalyze the nucleophile-assisted formation of C-C bonds is extremely small, and there is presently no definitive example of a biological Rauhut-Currier reaction. Biosynthesis of the polyketide insecticide spinosyn A in Saccharopolyspora spinosa involves a [4 + 2]-cycloaddition and a subsequent intramolecular C-C bond formation catalyzed by SpnF and SpnL, respectively. Isotope tracer experiments and kinetic isotope effects, however, imply that the SpnL-catalyzed reaction proceeds without initial deprotonation of the substrate. The crystal structure of SpnL exhibits high similarity to SAM-dependent methyltransferases as well as SpnF. The residue Cys60 is also shown to reside in the SpnL active site, and the Cys60Ala SpnL mutant is found to be devoid of activity. Moreover, SpnL is covalently modified at Cys60 and irreversibly inactivated when it is coincubated with a fluorinated substrate analogue designed as a suicide inactivator of nucleophile-assisted C-C bond formation. These results suggest that SpnL catalyzes a biological Rauhut-Currier reaction.

Evidence for an Enzyme-Catalyzed Rauhut-Currier Reaction during the Biosynthesis of Spinosyn A.,Choi SH, Jeon B, Kim N, Wu HH, Ko TP, Ruszczycky MW, Isiorho EA, Liu YN, Keatinge-Clay AT, Tsai MD, Liu HW J Am Chem Soc. 2021 Dec 8;143(48):20291-20295. doi: 10.1021/jacs.1c09482. Epub, 2021 Nov 23. PMID:34813308^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.