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8irm

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Endogenous substrate adenine bound state of Arabidopsis AZG1 at pH 5.5

Structural highlights

8irm is a 2 chain structure with sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 2.6Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

AZG1_ARATH Transports natural purines (adenine and guanine) as well as purine analogs. Confers sensitivity to 8-azaadenine and 8-azaguanine (8-azg).^[1]

Publication Abstract from PubMed

Cytokinins are essential for plant growth and development, and their tissue distributions are regulated by transmembrane transport. Recent studies have revealed that members of the 'Aza-Guanine Resistant' (AZG) protein family from Arabidopsis thaliana can mediate cytokinin uptake in roots. Here we present 2.7 to 3.3 A cryo-electron microscopy structures of Arabidopsis AZG1 in the apo state and in complex with its substrates trans-zeatin (tZ), 6-benzyleaminopurine (6-BAP) or kinetin. AZG1 forms a homodimer and each subunit shares a similar topology and domain arrangement with the proteins of the nucleobase/ascorbate transporter (NAT) family. These structures, along with functional analyses, reveal the molecular basis for cytokinin recognition. Comparison of the AZG1 structures determined in inward-facing conformations and predicted by AlphaFold2 in the occluded conformation allowed us to propose that AZG1 may carry cytokinins across the membrane through an elevator mechanism.

Structures and mechanisms of the Arabidopsis cytokinin transporter AZG1.,Xu L, Jia W, Tao X, Ye F, Zhang Y, Ding ZJ, Zheng SJ, Qiao S, Su N, Zhang Y, Wu S, Guo J Nat Plants. 2024 Jan;10(1):180-191. doi: 10.1038/s41477-023-01590-y. Epub 2024 , Jan 3. PMID:38172575^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Mansfield TA, Schultes NP, Mourad GS. AtAzg1 and AtAzg2 comprise a novel family of purine transporters in Arabidopsis. FEBS Lett. 2009 Jan 22;583(2):481-6. PMID:19121308 doi:10.1016/j.febslet.2008.12.048
↑ Xu L, Jia W, Tao X, Ye F, Zhang Y, Ding ZJ, Zheng SJ, Qiao S, Su N, Zhang Y, Wu S, Guo J. Structures and mechanisms of the Arabidopsis cytokinin transporter AZG1. Nat Plants. 2024 Jan 3. PMID:38172575 doi:10.1038/s41477-023-01590-y