8y2h
From Proteopedia
GK tetramer of AtP5CS1 filament with adjacent hooks, reaction state
Structural highlights
FunctionP5CS1_ARATH P5CS plays a key role in proline biosynthesis, leading to osmoregulation in plants. Publication Abstract from PubMedIn plants, the rapid accumulation of proline is a common response to combat abiotic stress(1-7). Delta-1-pyrroline-5-carboxylate synthase (P5CS) is a rate-limiting enzyme in proline synthesis, catalysing the initial two-step conversion from glutamate to proline(8). Here we determine the first structure of plant P5CS. Our results show that Arabidopsis thaliana P5CS1 (AtP5CS1) and P5CS2 (AtP5CS2) can form enzymatic filaments in a substrate-sensitive manner. The destruction of AtP5CS filaments by mutagenesis leads to a significant reduction in enzymatic activity. Furthermore, separate activity tests on two domains reveal that filament-based substrate channelling is essential for maintaining the high catalytic efficiency of AtP5CS. Our study demonstrates the unique mechanism for the efficient catalysis of AtP5CS, shedding light on the intricate mechanisms underlying plant proline metabolism and stress response. Dynamic Arabidopsis P5CS filament facilitates substrate channelling.,Guo CJ, Zhang T, Leng Q, Zhou X, Zhong J, Liu JL Nat Plants. 2024 May 13. doi: 10.1038/s41477-024-01697-w. PMID:38740943[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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