1ob8

Structural highlights

Function

HJE_SACS2 A structure-specific endonuclease that resolves Holliday junction (HJ) intermediates during genetic recombination. Acts only on 4-way DNA junctions in a sequence non-specific manner; introduces paired nicks in opposing strands 2 bases 3' of the point of strand exchange only on continuous strands of 4-way junction DNA. Cleaves both mobile and immobile junctions.^{[1] [2] [3]}

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

• Resolvase 3D structures

References

1. ↑ Kvaratskhelia M, White MF. An archaeal Holliday junction resolving enzyme from Sulfolobus solfataricus exhibits unique properties. J Mol Biol. 2000 Jan 14;295(2):193-202. PMID:10623519 doi:http://dx.doi.org/10.1006/jmbi.1999.3363
2. ↑ Kvaratskhelia M, White MF. Two Holliday junction resolving enzymes in Sulfolobus solfataricus. J Mol Biol. 2000 Apr 7;297(4):923-32. PMID:10736227 doi:http://dx.doi.org/10.1006/jmbi.2000.3624
3. ↑ Parker JL, White MF. The endonuclease Hje catalyses rapid, multiple turnover resolution of Holliday junctions. J Mol Biol. 2005 Jul 1;350(1):1-6. PMID:15921693 doi:http://dx.doi.org/10.1016/j.jmb.2005.04.056