1qno

Structural highlights

Function

MANA_HYPJR Endo-1,4-mannanase that catalyzes the random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans and heteromannans. It is a crucial enzyme for depolymerization of seed galactomannans and wood galactoglucomannans. Active against locust bean gum and ivory nut mannan, releasing mainly tri- and disaccharides (PubMed:7793911, Ref.3, Ref.4, PubMed:8529653). Also has transglycosylation activity. Transglycosylation of two mannotrioses into a mannohexaose is the major transglycosylation route (PubMed:8529653, Ref.7, PubMed:24950755).^{[1] [2] [3]} [PROSITE-ProRule:PRU00597][SAM:MobiDB-lite]^[4]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

• Mannosidase 3D structures

References

1. ↑ Rosengren A, Reddy SK, Sjöberg JS, Aurelius O, Logan DT, Kolenová K, Stålbrand H. An Aspergillus nidulans β-mannanase with high transglycosylation capacity revealed through comparative studies within glycosidase family 5. Appl Microbiol Biotechnol. 2014 Dec;98(24):10091-104. PMID:24950755 doi:10.1007/s00253-014-5871-8
2. ↑ Stålbrand H, Saloheimo A, Vehmaanperä J, Henrissat B, Penttilä M. Cloning and expression in Saccharomyces cerevisiae of a Trichoderma reesei beta-mannanase gene containing a cellulose binding domain. Appl Environ Microbiol. 1995 Mar;61(3):1090-7. PMID:7793911 doi:10.1128/aem.61.3.1090-1097.1995
3. ↑ Harjunpää V, Teleman A, Siika-Aho M, Drakenberg T. Kinetic and stereochemical studies of manno-oligosaccharide hydrolysis catalysed by beta-mannanases from Trichoderma reesei. Eur J Biochem. 1995 Nov 15;234(1):278-83. PMID:8529653 doi:10.1111/j.1432-1033.1995.278_c.x
4. ↑ Rosengren A, Reddy SK, Sjöberg JS, Aurelius O, Logan DT, Kolenová K, Stålbrand H. An Aspergillus nidulans β-mannanase with high transglycosylation capacity revealed through comparative studies within glycosidase family 5. Appl Microbiol Biotechnol. 2014 Dec;98(24):10091-104. PMID:24950755 doi:10.1007/s00253-014-5871-8